alexa Detection of free and immune-complexed serine protease and its antibody in TB patients with and without HIV co-infection.
Microbiology

Microbiology

Mycobacterial Diseases

Author(s): Shende N, Gupta S, Bhatia AS, Kumar S, Harinath BC

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Abstract OBJECTIVE: To understand the usefulness of detecting tuberculous IgG antibodies against mycobacterial excretory-secretory 31 kDa serine protease antigen (SEVA TB ES-31) and circulating free and circulating immune-complexed (CIC) serine protease in TB patients with and without HIV infection. DESIGN: Serum was collected from 144 individuals: patients with TB, with TB-HIV co-infection and HIV infection only, and ill and healthy controls. SEVA TB ES-31 antigen, a serine protease isolated from Mycobacterium tuberculosis H37Ra culture fluid, was used in indirect penicillinase ELISA to detect tuberculous antibodies. Similarly, affinity purified anti-ES-31 antibody was used in sandwich ELISA to detect circulating free and CIC serine protease. RESULTS: There was less sensitivity for tuberculous antibody in HIV-infected TB patients (46\%) than in those with TB alone (87\%) using mycobacterial serine protease. However, the sensitivity of detection of TB in the presence of HIV increased to 87\% by concomitant detection of circulating free and CIC serine protease antigen. CONCLUSION: Detection of free and CIC tuberculous serine protease antigen along with antibody is more useful for detecting TB in the presence of HIV co-infection.
This article was published in Int J Tuberc Lung Dis and referenced in Mycobacterial Diseases

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