alexa Detection of HIV-1 sequences in intestinal bacteria of HIV AIDS patients.


Journal of Antivirals & Antiretrovirals

Author(s): Zajac V, Stevurkova V, Matelova L, Ujhazy E

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Abstract OBJECTIVES: Bacterial DNA isolated from the intestinal tract of 11 American and 30 Slovak HIV/AIDS patients were analyzed by colony and dot blot hybridization assay for HIV-1 specific sequences. Secondly, PCR using primers specific for the HIV-1 gag, pol and env genes for detection of HIV-1 sequences in these DNA were performed. RESULTS: Intestinal bacteria DNA of HIV/AIDS patients hybridized in colony and dot blot hybridization assay for HIV-1 specific sequences. PCR products synthesized on specific primers of HIV-1 gag (115 bp), env (142 bp), pol-env (1484) genes were found to be for more than 90\% homologous to the corresponding sequence in HIV-1. CONCLUSIONS: Intestinal bacteria of HIV/AIDS patients are bearing sequences for more than 90\% identical with those of HIV-1.
This article was published in Neuro Endocrinol Lett and referenced in Journal of Antivirals & Antiretrovirals

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