alexa Detection of residual biocides in antibody drug conjugates for ImmunoPET imaging.


Immunotherapy: Open Access

Author(s): Colin D Medley, Jason Gruenhagen, Peter Yehl, Nik P Chetwyn

Abstract Share this page

Antibody drug conjugates are comprised of monoclonal antibodies conjugated to a small molecule drug to combine the targeted delivery of the antibody with the mechanism of action of the small molecule component. One class of antibody drug conjugates is used in ImmunoPET and combines the selectivity of the antibody with a positron emission tomography imaging agent that enables imaging tumors based on binding of the antibody. As the positron emission tomography imaging reagents are limited by their radioactive half-life, they are prepared at a single patient GMP quality scale with limited advance notification of when a patient requires the dose. One aspect of this single batch manufacture is using PD-10 columns to purify the ImmunoPET agent from the excess radioactive material used in the manufacturing process. These columns are kept in a storage solution containing biocides, 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, which prevent microbial growth in the column but could also adversely affect the patient if injected. We have developed a validatable HPLC-UV/MS method for the detection of biocides in formulated ImmunoPET drug product samples and formulation buffer. This method enables the demonstration of the clearance of biocides in the columns prior to use and quantitation of any residual biocides present in the drug product. This article was published in Analytical Methods and referenced in Immunotherapy: Open Access

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version