alexa Determination of aflatoxins B1, B2, G1 and G2 in spices using a multifunctional column clean-up.
Biomedical Sciences

Biomedical Sciences

Journal of Biomolecular Research & Therapeutics

Author(s): Akiyama H, Goda Y, Tanaka T, Toyoda M

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Abstract A rapid and simple method using a multifunctional column, which contains lipophilic and charged active sites, was developed to analyse aflatoxins B1, B2, G1 and G2 in various spices, such as red pepper and nutmeg. After extraction by acetonitrile:water (9:1) and clean-up using MultiSep #228 column, the aflatoxins and aflatoxin-TFA derivatives are determined using LC with fluorescence detection. Recoveries of each aflatoxin B1, B2, G1 and G2 spiked to red pepper, white pepper, black pepper, nutmeg and tear grass at the level of 10 ng/g were over 80-85\% in all instances. The minimum detectable concentration for aflatoxins in red pepper was 0.5 ng/g.
This article was published in J Chromatogr A and referenced in Journal of Biomolecular Research & Therapeutics

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