alexa Determination of geosmin, 2-methylisoborneol, and a musty-earthy odor in wheat grain by SPME-GC-MS, profiling volatiles, and sensory analysis.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Jele HH, Majcher M, ZawirskaWojtasiak R, Wiewirowska M, Wasowicz E

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Abstract Geosmin and 2-methylisoborneol-compounds responsible for the musty-earthy off-odor of wheat grain, were isolated by SPME and analyzed by GC-MS. Carboxen/PDMS/DVB fiber coating was selected because of its highest extraction efficiency. Concentrations of geosmin and 2-methylisoborneol as low as 0.001 microg/kg were detected in SIM mode using ion trap mass spectrometer. Apart from GC-MS determination of geosmin and 2-methylisoborneol, various methods for evaluating the musty-earthy off-odor caused by these compounds in wheat grain are presented. Sensory profile analysis differentiated wheat grain into sound and off-flavored, but the method was tedious. Similar groupings, however, were obtained using more rapid methods such as comparison of volatile profiles using SPME-fast GC with PCA projection of data and metal oxide (MOS) based electronic nose. This article was published in J Agric Food Chem and referenced in Journal of Chromatography & Separation Techniques

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