alexa DETERMINATION OF GUAIFENE SIN AND DEXTROMETHORPHAN IN A COUGH SYRUP BY HPLC WI TH FLUOROMET RIC DETECTION
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Abdil ZDEMIR, Halil AKSOY, Erdal DIN, Dumitru BLEANU, Saadet DERMI

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A practical high-performance liquid chromatographic method with fluorometric detection for the determination of guaifenesin and dextromethorphan in cough syrup is described. T he developed method contains simple, accurate and rapid sample preparation steps for the separation and simultane ous determination of active compounds in samples with existence of other excipients. Amilorid (AM) was used as a n internal standard (IS) that has a fluorescence character in the working wavelength region. A good chromatograp hic separation of two drugs and IS was achieved on a Waters Symmetry ® C18 Column 5 μm 4.6 × 250 mm with mobile ph ase consisting of phosphate buffer (0.2 M, pH=2), acetonitrile and methyl alcohol (v/v, 62:23:15). Eluents were monitored by fluorescence detection at the excitation and emission wavelengths of 277 and 588 nm, resp ectively. Under the described conditions, the obtained calibration graphs are l inear over the concentration r ange of 0.05–0.2 and 1.2–2.4 mg/mL for dextromethorphan and guaifenesin, respectively and the regression coefficients w ere greater than 0.999. The validation study was carried out fulfilling the ICH guidelines in order to prove tha t the new analytical method meets the fundamental criteria including selectivity, linearity, precision, accuracy and sensitivity

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This article was published in Revue Roumaine de Chimie and referenced in Journal of Chromatography & Separation Techniques

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