alexa Determination of halofuginone in bovine plasma by competing-ion high performance liquid chromatography after solid phase extraction.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Kinabo LD, McKellar QA, Murray M

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Abstract A high performance liquid chromatography (HPLC) method for the determination of the anticoccidial and antitheilerial drug halofuginone in bovine plasma was developed. Samples were diluted with acetic acid (10\%, v/v) and cleaned up on a Bond Elut C8 column. The analyte was eluted from the extraction column and chromatographed by reversed-phase HPLC using decylamine as a competing-ion reagent. Detection was by UV at 243 nm. Recovery from plasma was 75\%, and within-day and between-day coefficients of variation were 5.23 and 6.35\% respectively. The specificity and sensitivity of this method (limit of detection in plasma, 1 ng/mL) were sufficiently high to enable us to characterize the time course of the drug in plasma after oral administration of therapeutic doses to cattle. This article was published in Biomed Chromatogr and referenced in Journal of Chromatography & Separation Techniques

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