alexa Determination of leucovorin and 5-fluorouracil in plasma by high-performance liquid chromatography.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Vandenbosch C, van Belle S, de Smet M, Taton G, Bruynseels V,

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Abstract A method was developed for the determination of (6R)- and (6S)-leucovorin and 5-fluorouracil in plasma. As leucovorin diastereoisomers cannot be separated on a classical reversed-phase column, it was necessary to use a chiral stationary phase. The method presented is based on the same principle as the method described by Wainer and Stiffin [J. Chromatogr., 424 (1988) 158], i.e., coupling of a bovine serum albumin phase to an achiral stationary phase. Before the chromatography, the drug was isolated from the plasma matrix by solid-phase extraction. For 5-fluorouracil, chromatography was performed on a classical RP-18 column after extraction from the plasma by liquid-liquid extraction. Both methods were validated and applied to the analysis of patients' samples.
This article was published in J Chromatogr and referenced in Journal of Chromatography & Separation Techniques

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