alexa Determination of Major Bovine Milk Proteins by Reversed Phase High Performance Liquid Chromatography
Food & Nutrition

Food & Nutrition

Advances in Dairy Research

Author(s): Juan WANG, QingHe ZHANG, ZhiHua WANG, HongMei LI

Abstract Share this page

A reversed-phase high-performance liquid chromatography (RP-HPLC) for the quantification of six major bovine milk proteins (κ-casein (κ-CN), αs2-casein (αs2-CN), αs1-casein (αs1-CN), β-casein (β-CN), immunoglobulins (IgG) and whey) was developed. The separation and quantification of these major proteomic components were achieved with an Agilent Zorbax 300SB-C8 analytical column (250 mm × 4.6 mm I.D., 5 μm) at 45 °C with detection wavelength at 214 nm. The gradient elution was carried out with solvents of 0.1% TFA in water and 0.1% TFA in acetonitrile at a flow rate of 0.8 ml min−1. The method validation consisted of testing the linearity, repeatability, reproducibility and accuracy. The recoveries of six target proteins spiked in milk were in the range from 74.8% to 132.5%. Nine different commercial milk brands were used, the difference of the concentration and relative ration of κ-CN, αs2-CN, αs1-CN, β-CN and whey were found.

This article was published in Chinese Journal of Analytical Chemistry and referenced in Advances in Dairy Research

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version