alexa Determination of the degree of deacetylation of chitin and chitosan by X-ray powder diffraction.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Molecular and Genetic Medicine

Author(s): Zhang Y, Xue C, Xue Y, Gao R, Zhang X

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Abstract A new method to determine the degree of deacetylation (DD) of alpha-chitin and chitosan in the range of 17-94\% DD using X-ray powder diffraction (XRD) is proposed. The results were calibrated using (1)H NMR spectroscopy for chitosan and FTIR for chitin, in comparison with the potentiometric titration method. The results showed a good linear correlation between the CrI020 from XRD and the calibrated DD value. This method is found to be simple, rapid and nondestructive to the sample. This article was published in Carbohydr Res and referenced in Journal of Molecular and Genetic Medicine

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