Author(s): Herv C, Beyne P, Jamault H, Delacoux E
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Abstract An isocratic reversed-phase high-performance liquid chromatographic method for the simultaneous determination of tryptophan and four metabolites of the kynurenine pathway (kynurenine, 3-hydroxykynurenine, kynurenic acid and 3-hydroxyanthranilic acid) in human serum is described. This new method, which uses both isocratic elution and two on-line connected programmable ultraviolet and spectrofluorimetric detectors, allows the determination of these metabolites, in the physiological ranges, with satisfying specificity and sensitivity within 30 min.
This article was published in J Chromatogr B Biomed Appl
and referenced in Journal of Analytical & Bioanalytical Techniques