alexa Detoxification of corn stover prehydrolyzate by trialkylamine extraction to improve the ethanol production with Pichia stipitis CBS 5776.
Chemical Engineering

Chemical Engineering

Journal of Bioprocessing & Biotechniques

Author(s): Zhu J, Yong Q, Xu Y, Yu S

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Abstract In order to realize the separated ethanol fermentation of glucose and xylose, prehydrolysis of corn stover with sulfuric acid at moderate temperature was applied, while inhibitors were produced inevitably. A complex extraction was adopted to detoxify the prehydrolyzate before fermentation to ethanol with Pichia stipitis CBS 5776. The best proportion of mixed extractant was 30\% trialkylamine-50\% n-octanol -20\% kerosene. Detoxification results indicated that 73.3\% of acetic acid, 45.7\% of 5-hydroxymethylfurfural and 100\% of furfural could be removed. Compared with the undetoxified prehydrolyzate, the fermentability of the detoxified prehydrolyzate was significantly improved. After 48 h fermentation of the detoxified prehydrolyzate containing 7.80 g/l of glucose and 52.8 g/l of xylose, the sugar utilization ratio was 93.2\%; the ethanol concentration reached its peak value of 21.8 g/l, which was corresponding to 82.3\% of the theoretical value. Copyright © 2010 Elsevier Ltd. All rights reserved. This article was published in Bioresour Technol and referenced in Journal of Bioprocessing & Biotechniques

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