alexa Development and evaluation of a molecular assay for detection of nontuberculous mycobacteria by use of the cobas amplicor platform.
Microbiology

Microbiology

Journal of Medical Microbiology & Diagnosis

Author(s): PeterGetzlaff S, Lthy J, Bddinghaus B, Bttger EC, Springer B

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Abstract We have developed and evaluated a semiautomated assay for detection of nontuberculous mycobacteria (NTM) from clinical samples based on the Cobas Amplicor Mycobacterium tuberculosis test (Roche Diagnostics, Switzerland). A capture probe, specific for mycobacteria at the genus level, was linked to magnetic beads and used for the detection of amplification products obtained by the Cobas Amplicor M. tuberculosis assay. We demonstrate that the analytical sensitivity of the genus assay is similar to that of Cobas Amplicor M. tuberculosis detection. Four hundred sixteen clinical specimens were evaluated for the presence of NTM DNA. Sensitivities for smear-positive and smear-negative specimens were found to be 100\% and 47.9\%, respectively. Specificity was 97.7\%, the positive predictive value 84.6\%, and the negative predictive value 93.1\%. The genus assay is easy to perform, produces reliable results, and was found to be a valuable diagnostic tool for rapid diagnosis of infections with NTM. The genus assay has the potential to detect NTM not routinely recovered by culture and to discover new mycobacterial species.
This article was published in J Clin Microbiol and referenced in Journal of Medical Microbiology & Diagnosis

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