Author(s): Patil Sudarshan S, Bonde C G
Simple spectrophotometric method has been developed for simultaneous estimation of Glibenclamide and Metformin HCl in combined dosage form. The method employed simultaneous equation method for analysis using methanol as a solvent. The two wavelengths 229.5 nm and 237 nm were selected for estimation of Glibenclamide and Metformin HCl respectively. Linearity was observed in the concentration range of 3-15 μ g/ml and 2-10 μ g/ml for Glibenclamide and Metformin HCl respectively. The recovery studies ascertained the accuracy of the proposed method and the results were validated as per ICH guidelines. The method can be employed for estimation of pharmaceutical formulations with no interference from any other excipients and diluents.