alexa Development and validation of liquid chromatographic and UV derivative spectrophotometric methods for the determination of Metformin, Pioglitazone and Glimepiride in pharmaceutical formulations.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): KS Lakshmi, T Rajesh, S Sharma, S Lakshmi

Abstract Share this page

A high-performance liquid chromatographic method and a UV derivative spectrophotometric method for the simultaneous determination of metformin (MFN), pioglitazone (PLZ) and glimepiride (GLM), in tablets were developed in the present work. The various parameters, such as linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation were studied according to International Conference on Harmonization guidelines. HPLC was carried out by using the reversed-phase technique on an phenomenex RP-18 column (150x 4.6mm, 5µ ) with a mobile phase consisted of acetonitrile and phosphate buffer (pH 3) in the ratio of 65: 35. The flow rate was fixed at 0.5ml/min and the drugs were monitored at 245nm with UV dual absorbance detector and the elution time was found less than 10 min indicates shorter analysis time. The first derivative UV spectrophotometric method was performed at 260.1, 280.7 and 251.5nm for MFN, PLZ and GLM respectively. Statistical analysis was done by Student’s t-test and F-test, which showed no significant difference between the results obtained by the two methods. The proposed methods are highly sensitive, precise and accurate and therefore can be used for its intended purpose.

  • To read the full article Visit
  • Subscription
This article was published in Online Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry and referenced in Journal of Chromatography & Separation Techniques

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords