alexa Development of a novel Agrobacterium-mediated transformation method to recover transgenic Brassica napus plants.


Journal of Antivirals & Antiretrovirals

Author(s): Wang WC, Menon G, Hansen G

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Abstract We report here an in planta method to produce transgenic Brassica napus plants. The procedure included Agrobacterium-mediated inoculation of plants at various development stages along with a vacuum infiltration step. The flowering stage appeared to be the most receptive stage for transformation and production of transgenic plants. In some cases, the flowering stage was induced either by cold treatment or by high density planting. Molecular and genetic analysis revealed that single and multiple copy events were generated and that the transgenes were transmitted to the T1 and T2 progeny in a Mendelian fashion. This article was published in Plant Cell Rep and referenced in Journal of Antivirals & Antiretrovirals

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