alexa Development of a novel treatment for leukemia directed at tumor-associated mRNA splicing.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Stem Cell Research & Therapy

Author(s): Daines DA, Sun J, Uchakina ON, McKallip RJ

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Abstract This report describes a novel approach to cancer therapy that targets genes that are preferentially alternatively spliced and expressed in leukemia. We developed CD44v6 and CD44v8 splicing constructs fused with GFP or a humanized fragment of Pseudomonas aeruginosa exotoxin A (hPE24). Transfection of K562 leukemia cells with the GFP-linked splicing constructs led to subsequent production of detectable levels of GFP. Transfection of K562 cells with the hPE24-linked splicing constructs led to significant reduction of cell viability and an increase in the induction of apoptosis. Normal human PBMCs were unaffected by following transfection with these constructs. Copyright © 2013 Elsevier Ltd. All rights reserved. This article was published in Leuk Res and referenced in Journal of Stem Cell Research & Therapy

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