Author(s): Schiewe MC, Neev J, Hazeleger NL, Balmaceda JP, Berns MW,
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Abstract The purpose of this study was to assess the efficacy of the holmium:yttrium scandian gallium garnet (Ho:YSGG) laser, operating in a pipette-free, non-contact mode, to assist hatching and sustain normal embryonic development. Two-cell mouse embryos were recovered and assigned to laser-assisted hatching (LAH) treatment or control human tubal fluid (HTF) culture with or without serum (HTF-s, HTF-o) or with late serum supplementation (HTF-o/s). The basic experimental apparatus for LAH consisted of a stationary 2.1 microns Ho:YSGG laser beam directed through a mechanical shutter into an input port of a Zeiss Axiomat inverted microscope. Fewer (P < 0.05) embryos developed to the blastocyst stage in the HTF-s group (81\%) than in the LAH (90\%), HTF-o (94\%) and HTF-o/s (92\%) groups. The level of hatching was significantly increased (P < 0.01) after the LAH treatment (57\%) compared to HTF-o/s (32\%), HTF-s (18\%) or HTF-o (5\%). Implantation rates were not significantly impaired following the LAH treatment (21\%). These data demonstrate that LAH using the Ho:YSGG laser is a simple, accurate and effective procedure for assisted hatching.
This article was published in Hum Reprod
and referenced in Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biology