Author(s): Burt JP, Pethig R, Gascoyne PR, Becker FF
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Abstract Dielectrophoresis measurements, the study of the motion of particles in non-uniform a.c. electrical fields, have been made on three cell lines (DS19, R1 and DR1) of Friend murine erythroleukaemia cells as a function of hexamethylene bisacetamide (HMBA) treatment. The effects of saponin treatment on R1 cells and neuraminidase on human red blood cells were also studied. It is shown that the dielectrophoretic behaviour can be interpreted in terms of cell surface charge and cell membrane conductivity effects. HMBA reduces the cell surface charge on all three cell lines, and in lines DS19 and DR10, where the cells are induced to differentiate, there is an increase in effective cell conductivity. This gain in conductivity is concluded to be associated with either an enhanced lateral electrophoretic motion of delocalised ions or of the polarisability of dipoles at the membrane surface.
This article was published in Biochim Biophys Acta
and referenced in Journal of Membrane Science & Technology