alexa Differential expression of iNOS and cNOS mRNA in human vascular smooth muscle cells and endothelial cells under normal and inflammatory conditions.


Translational Medicine

Author(s): MacNaul KL, Hutchinson NI

Abstract Share this page

Abstract To examine the potential contribution of endothelial cell cNOS (ec-cNOS) and inducible NOS (iNOS) in controlling vascular tone under normal versus inflammatory conditions, we performed Northern hybridizations to examine the differential expression of each NOS mRNA in human aortic endothelial cells (AOEC) and human aortic smooth muscle cells (AOSMC) cultured for 8 h in the presence or absence of cytokines (IL-1 beta, TNF-alpha, and IFN-gamma) and LPS. Cytokine/LPS treatment induced a 4.4 kb iNOS mRNA in the human AOSMC; in contrast, cytokine/LPS treatment down regulated the expression of ec-cNOS mRNA in the AOEC. No iNOS mRNA was detected in the AOEC under the conditions examined. These results suggest that under specific inflammatory conditions the generation of NO in vascular tissue switches from ec-cNOS in the endothelium to iNOS in the smooth muscle. This article was published in Biochem Biophys Res Commun and referenced in Translational Medicine

Relevant Expert PPTs

Relevant Speaker PPTs

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version