alexa Differential Regulation of High Glucose–Induced Glyceraldehyde-3-Phosphate Dehydrogenase Nuclear Accumulation in Müller Cells by IL-1β and IL-6
Ophthalmology

Ophthalmology

Journal of Clinical & Experimental Ophthalmology

Author(s): Yego EC, Vincent JA, Sarthy V, Busik JV, Mohr S

Abstract Share this page

purpose. This study determined the role of the proinflammatory cytokines known to be elevated in the diabetic retina, namely IL-1β, TNFα, and IL-6, in a high glucose–induced nuclear accumulation of GAPDH in retinal Müller cells, an event considered crucial for the induction of cell death. methods. With use of the transformed rat Müller cell line (rMC-1) and isolated human Müller cells (HMCs), the authors examined the effect of high glucose (25 mM), IL-1β, TNFα, IL-6, and high glucose (25 mM) plus inhibitors of the caspase-1/IL-1β signaling pathway on GAPDH nuclear accumulation, which was evaluated by immunofluorescence analysis. results. High glucose induced IL-1β, weak IL-6, and no TNFα production by rMC-1 and HMCs. IL-1β (1–10 ng/mL) significantly increased GAPDH nuclear accumulation in Müller cells in a concentration-dependent manner within 24 hours. Further, high glucose–induced GAPDH nuclear accumulation in Müller cells was mediated by IL-1β. Inhibition of the IL-1 receptor using an IL-1 receptor antagonist (IL-1ra; 50 ng/mL) or inhibition of IL-1β production using a specific caspase-1 inhibitor (YVAD-fmk; 100 μM) significantly decreased high glucose–induced GAPDH nuclear accumulation. In contrast, IL-6 (2 ng/mL) had a strong protective effect attenuating high glucose and IL-1β–induced GAPDH nuclear accumulation in Müller cells. TNFα (1–10 ng/mL) did not have any effect on GAPDH nuclear accumulation. conclusions. These results revealed a novel mechanism for high glucose–induced GAPDH nuclear accumulation in Müller cells through production and autocrine stimulation by IL-1β. The protective role of IL-6 in high glucose– and IL-1β–induced toxicity indicates that changes in the balance of these cytokines might contribute to cellular damage mediated by elevated glucose levels.

This article was published in Investigative Ophthalmology & Visual Science and referenced in Journal of Clinical & Experimental Ophthalmology

Relevant Expert PPTs

Relevant Speaker PPTs

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords