Author(s): Waites KB, Brookings ES, Moser SA, Zimmer BL
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Abstract Studies were conducted on a method of direct inoculation of MicroScan overnight and rapid panels from positive BacT/Alert blood culture bottles containing standard aerobic media to determine the correlation with inoculation of the corresponding panels with a standardized bacterial suspension obtained following subculture to agar. For Gram-negative organisms, 122 of 127 (96\%) overnight panels and 85 of 118 (72\%) rapid panels showed complete agreement with the standard method for species identification. Highest concordance (99\%) occurred with Enterobacteriaceae inoculated directly into overnight panels. For Gram-positive organisms, 70 of 85 (82\%) overnight panels and 45 of 86 (52\%) rapid panels showed complete agreement. These findings suggest that direct inoculation of Gram-negative overnight MicroScan panels yields results most comparable to standard methods when Enterobacteriaceae are detected and allows reporting of results 18 to 24 h sooner. Direct inoculation of Gram-positive overnight or rapid panels and Gram-negative rapid panels from this blood culture medium did not yield acceptable identification results and is not recommended.
This article was published in Diagn Microbiol Infect Dis
and referenced in Mass Spectrometry & Purification Techniques