alexa Direct electron transfer of glucose oxidase and biosensing of glucose on hollow sphere-nanostructured conducting polymer metal oxide composite.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): Guo CX, Li CM

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Abstract A hollow sphere-nanostructured conductive polymer/metal oxide composite was synthesized and used to investigate the electrochemical behavior of glucose oxidase, demonstrating a significantly enhanced direct electron transfer ability of glucose oxidase. In particular, the long-standing puzzle of whether enzymatic glucose sensing involves an enzyme direct electron transfer process was studied. The results indicate the mechanism is indeed a glucose oxidase direct electron transfer process with competitive glucose oxidation and oxygen reduction to detect glucose. A glucose biosensor with the glucose oxidase-immobilized nanomaterial was further constructed, demonstrating superior sensitivity and reliability, and providing great potential in clinical applications. This article was published in Phys Chem Chem Phys and referenced in Journal of Microbial & Biochemical Technology

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