alexa Direct in vitro regeneration of lentil (Lens culinaris Medik.).
Psychiatry

Psychiatry

Journal of Addiction Research & Therapy

Author(s): Omran VG, Bagheri A, Moshtaghi N

Abstract Share this page

Abstract This study surveyed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting by different explants and different concentration of BAP. Due to optimization of shoot regeneration, two media including of MS and modified MS (MS salts with double concentration of CaC21, and B5 vitamins), different explants such as decapitated embryo axes, epicotyls and cotyledonary nodes and different concentrations of BAP hormone (1, 1.5, 2, 2.5, 3 and 4 mg L(-1)) in two genotypes (Gachsaran and Flip. 92-12 L) were used. The results showed that modified MS is a suitable medium for in vitro shoot regeneration of lentil. High levels of BAP caused increasing of shoot regeneration in lentil genotypes. Three milligram per liter of BAP induced the highest level of shoot regeneration. In addition, decapitated embryo explants were the best explants for highest shoot regeneration (5.8) (p < 0.05). However, increasing of hormone concentration from 2 to 3 and 4 mg L(-1) caused decreasing in the number of shoots, so 2 mg L(-1) of BAP was best. For rooting, the in vitro-in vivo method of rooting was better than only in vitro method. The shoots regenerated in 2 mg L(-1) BAP had higher rooting percentage than the shoots were regenerated in 3 and 4 mg L(-1) BAP. These results indicate on the inhibitory effect of high concentration of BAP on root induction. But the genotype didn't have any significant effect on rooting percentage and length of roots.
This article was published in Pak J Biol Sci and referenced in Journal of Addiction Research & Therapy

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords