Author(s): Shigechi H, Koh J, Fujita Y, Matsumoto T, Bito Y, , Shigechi H, Koh J, Fujita Y, Matsumoto T, Bito Y,
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Abstract Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis alpha-amylase by using the C-terminal-half region of alpha-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5\% of theoretical yield from raw corn starch.
This article was published in Appl Environ Microbiol
and referenced in Journal of Bioprocessing & Biotechniques