Author(s): Zannis VI, Cole FS, Jackson CL, Kurnit DM, Karathanasis SK
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Abstract Recently developed molecular probes for human apolipoprotein (apo) genes have been used to study the specificity of human tissue expression of the apo A-I, apo C-II, apo C-III, and apo E genes. We have found that apo E mRNA was present in all tissues examined. On the basis of total RNA concentration the relative abundance of apo E mRNA expressed as a percentage of the liver value is as follows: adrenal gland and macrophages, 74-100\%; gonads and kidney, 12-15\%; spleen, brain, thymus, ovaries, intestine, and pancreas, 3-9\%; heart, 1.5\%; stomach, striated muscle, and lung, less than 1\%. The relative concentration of apo E mRNA in cultures of human peripheral blood monocyte-macrophages increases dramatically as a function of time in culture, and after 5 days, it compares to that of liver. The human tissues shown to synthesize apo E mRNA were also examined for their ability to synthesize apo A-I, apo C-II, and apo C-III mRNA. The relative abundance of apo A-I, apo C-III, and apo C-II mRNA expressed as a percentage of the liver value is as follows: apo A-I, intestine, 50\%; apo A-I, pancreas and gonads, 12\%; apo A-I, kidney, 4\%; apo A-I, adrenal, 2.5\%; apo A-I, ovaries and heart, 1\%; apo A-I, stomach and thymus, less than 1\%; apo C-III, intestine, 62\%; apo C-III, pancreas, 7\%; apo C-II, intestine, 3\%; apo C-II, pancreas, less than 1\%. The knowledge of tissue specificities in the synthesis of apolipoproteins is important for our understanding of the regulation of apolipoproteins and lipoprotein metabolism.
This article was published in Biochemistry
and referenced in Journal of Proteomics & Bioinformatics