Author(s): Semagn K, Bjrnstad A, Skinnes H, Mary AG, Tarkegne Y,
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Abstract A genetic linkage mapping study was conducted in 93 doubled-haploid lines derived from a cross between Triticum aestivum L. em. Thell 'Arina' and a Norwegian spring wheat breeding line, NK93604, using diversity arrays technology (DArT), amplified fragment length polymorphism (AFLP), and simple sequence repeat (SSR) markers. The objective of this study was to understand the distribution, redundancy, and segregation distortion of DArT markers in comparison with AFLP and SSR markers. The map contains a total of 624 markers with 189 DArTs, 165 AFLPs and 270 SSRs, and spans 2595.5 cM. All 3 marker types showed significant (p < 0.01) segregation distortion, but it was higher for AFLPs (24.2\%) and SSRs (22.6\%) than for DArTs (13.8\%). The overall segregation distortion was 20.4\%. DArTs showed the highest frequency of clustering (27.0\%) at < 0.5 cM intervals between consecutive markers, which is 3 and 15 times higher than SSRs (8.9\%) and AFLPs (1.8\%), respectively. This high proportion of clustering of DArT markers may be indicative of gene-rich regions and (or) the result of inclusion of redundant clones in the genomic representations, which was supported by the presence of very high correlation coefficients (r > 0.98) and multicollinearity among the clustered markers. The present study is the first to compare the utility of DArT with AFLP and SSR markers, and the present map has been successfully used to identify novel QTLs for resistance to Fusarium head blight and powdery mildew and for anther extrusion, leaf segment incubation, and latency.
This article was published in Genome
and referenced in Journal of Molecular Biomarkers & Diagnosis