alexa Distribution of the endothelial constitutive nitric oxide synthase in the developing rat brain: an immunohistochemical study.
Pharmaceutical Sciences

Pharmaceutical Sciences

Journal of Pharmacological Reports

Author(s): Tpel I, Stanarius A, Wolf G

Abstract Share this page

Abstract The present study deals with the distribution of endothelial constitutive nitric oxide synthase (ecNOS) in the developing rat brain using optimized protocols for preparation and fixation and the tyramide-signal-amplification technique. The immunostaining patterns of a monoclonal antibody against ecNOS are compared with results obtained with a rat pan-endothelial marker, the monoclonal RECA-1 antibody. It is shown that ecNOS is present in the endothelial lining of all types of blood vessels and the choroid plexuses of the rat brain from the beginning of vasculogenesis at embryonic day 11 until adulthood (75 weeks). The same is true for RECA-1 immunoreactivity, that was demonstrated in the developmental brain vasculature for the first time. Both antigens expressed identical immunostaining patterns. At all investigated stages of brain development neither ecNOS negative blood vessels nor ecNOS positive non-endothelial cells, e.g., neurons, were found. The data indicate that ecNOS is involved in the embryonic angiogenesis and the regulation of hemodynamic functions of brain vasculature throughout the individual life. Copyright 1998 Elsevier Science B.V.
This article was published in Brain Res and referenced in Journal of Pharmacological Reports

Relevant Expert PPTs

Relevant Speaker PPTs

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords