alexa DNA damage caused by common cytological fixatives.
Medicine

Medicine

Advanced Techniques in Biology & Medicine

Author(s): Douglas MP, Rogers SO, Douglas MP, Rogers SO

Abstract Share this page

Abstract Tissues from nine species of plants and fungi were treated separately with eight solutions, including seven cytological fixatives (3.7\% formaldehyde at pH 3.0 and 7.0, FAA at pH 3.0 and 7. 0, 1\% glutaraldehyde at pH 3.0 and 7.0, and Lavdowsky's fluid at pH 3.0) and one storage buffer (SED=NaCl-EDTA-DMSO, pH 7.0). DNA from untreated tissue and SED-treated tissue was of high molecular weight (>50 kb). DNA from glutaraldehyde-treated tissues averaged 20 kb in length, while DNA from all other treatments averaged less than 8 kb in length. Each DNA was subjected to amplification using the polymerase chain reaction, followed by sequencing of 250 bp near the 3' end of the nuclear rRNA small subunit gene. Glutaraldehyde treatments (at pH 3.0 and 7.0) produced damaged bases at rates of 0. 0\% to less than 0.1\%. Treatments with Lavdowsky's fluid (containing mercuric chloride), FAA at pH 7.0, and SED produced rates of 0.0\% to 3.6\%. FAA at pH 3.0 produced rates of 7.6\% to 15.6\%. Nearly 100 attempts to amplify from specimens treated with 3.7\% formaldehyde (at pH 3.0 and 7.0) failed, indicating extreme damage to the DNA. Copyright 1998 Elsevier Science B.V. All rights reserved.
This article was published in Mutat Res and referenced in Advanced Techniques in Biology & Medicine

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords