alexa DNA-induced aggregation and fusion of phosphatidylcholine liposomes in the presence of multivalent cations observed by the cryo-TEM technique
Microbiology

Microbiology

Journal of Chemical Biology & Therapeutics

Author(s): Kuvichkin VV, Danev RS, Shigematsu H, Nagayama K

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By means of cryoelectron transmission microscopy (cryo-TEM), we were able to demonstrate the formation of ternary complexes (TC): DNA-phosphatidylcholine liposome-divalent metal cations. Addition of Ba2+ to TC led to visualization of DNA compacting on the liposome surface. Staining the TC by Tb3+ cations revealed the changed secondary structure of DNA located between fused liposomes. Cryo-TEM and liposome turbidity data were analyzed during TC formation. Liposome aggregation and the liposome fusion induced by DNA in TC were observed. Because TC displayed the property of DNA cationic liposome complexes as well as their own unique properties, we were able to consider cationic lipoplexes as a particular case of TC. The involvement of TC and direct DNA-lipid interactions in the formation nuclear pore complexes were assumed.

This article was published in J Membr Biol and referenced in Journal of Chemical Biology & Therapeutics

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