alexa Dot-ELISA vs. PCR of fine needle aspirates of tuberculous lymphadenitis: a prospective study in India.
Infectious Diseases

Infectious Diseases

Journal of Infectious Diseases & Preventive Medicine

Author(s): Jain A, Verma RK, Tiwari V, Goel MM, Jain A, Verma RK, Tiwari V, Goel MM

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Abstract OBJECTIVE: To evaluate an in-house dot-enzyme-linked immunosorbent assay (ELISA) for confirmation of clinically suspected cases of tuberculous lymphadenitis (TBLN). STUDY DESIGN: The study was performed at the postgraduate departments of microbiology and pathology of a tertiary-care teaching hospital in India. Suspected cases of TBLN were prospectively enrolled. Fine needle aspiration was done of enlarged lymph nodes in all patients, and 2 smears were prepared, 1 for acid-fast bacillus (AFB) demonstration and the other for cytologic examination. The remaining material was tested with in-house dot-ELISA and by IS6110 amplification with polymerase chain reaction (PCR), for diagnosis of TBLN. RESULTS: ELISA was more sensitive and detected 93.2\% of cases. PCR and fine needle aspiration cytology (FNAC) detected 82.5\% and 61.0\% cases, respectively. AFB positivity was 33.1\%. CONCLUSION: Application of dot-ELISA was more sensitive but less specific as compared to PCR. PCR, though expensive, should be used in problem cases because of its high specificity.
This article was published in Acta Cytol and referenced in Journal of Infectious Diseases & Preventive Medicine

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