alexa Effect of agitation and aeration on the production of nitrile hydratase by Rhodococcus erythropolis MTCC 1526 in a stirred tank reactor.
Microbiology

Microbiology

Fermentation Technology

Author(s): Kamble AL, Meena VS, Banerjee UC

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Abstract AIMS: To evaluate the effect of different physicochemical parameters such as agitation, aeration and pH on the growth and nitrile hydratase production by Rhodococcus erythropolis MTCC 1526 in a stirred tank reactor. METHODS AND RESULTS: Rhodococcus erythropolis MTCC 1526 was grown in 7-l reactor at different agitation, aeration and controlled pH. The optimum conditions for batch cultivation in the reactor were an agitation rate of 200 rev min(-1) , aeration 0.5 v/v/m at controlled pH 8. In this condition, the increase in nitrile hydratase activity was almost threefold compared to that in the shake flask. CONCLUSION: Agitation and aeration rate affected the dissolved-oxygen concentration in the reactor which in turn affected the growth and enzyme production. SIGNIFICANCE AND IMPACT OF THE STUDY: Cultivation of R. erythropolis MTCC 1526 in the reactor was found to have significant effect on the growth and nitrile hydratase production when compared to the shake flask. © 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology. This article was published in Lett Appl Microbiol and referenced in Fermentation Technology

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