alexa Effect of crowding and chaperones on self-association, aggregation and reconstitution of apophosphorylase b.


Biochemistry & Analytical Biochemistry

Author(s): Chebotareva NA, Eronina TB, Roman SG, Poliansky NB, Muranov KO,

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Abstract It was shown that the rate of reconstruction of muscle glycogen phosphorylase b (Phb) from apoenzyme and pyridoxal 5'-phosphate decreased under crowding conditions. The effect of crowding was counteracted by chaperones (α-crystallin and proline). Sedimentation analysis shows that crowding stimulates the formation of high-molecular-weight associates at 25 °C, whereas chaperones stabilize small oligomers. The study of the kinetics of apoPhb aggregation at 37 °C showed that the anti-aggregation activity of chaperones decreased under crowding conditions. When studying the sedimentation behavior of the mixture of apoPhb and α-crystallin, the complexes between unfolded apoPhb and dissociated forms of α-crystallin were observed. It is assumed that these complexes are responsible for realization of the chaperone-like activity of α-crystallin under crowding conditions. Copyright © 2013 Elsevier B.V. All rights reserved. This article was published in Int J Biol Macromol and referenced in Biochemistry & Analytical Biochemistry

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