alexa Effect of room fluorescent light on the deterioration of tissue culture medium.
Molecular Biology

Molecular Biology

Journal of Cytology & Histology

Author(s): Wang RJ

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Abstract A major cause of tissue culture medium deterioration is exposure to room fluorescent light. Riboflavin and tryptophan present in Dulbecco's modified Eagle's minimum essential medium, when exposed to light, yield toxic photoproducts responsible for loss of the ability of the medium to support clonal growth of human, mouse and Chinese hamster cell lines. Procedures for minimizing medium deterioration are discussed.
This article was published in In Vitro and referenced in Journal of Cytology & Histology

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