alexa Effective modulation of transforming growth factor-β1 expression through engineered microRNA-based plasmid-loaded nanospheres.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Stem Cell Research & Therapy

Author(s): Zhou Y, Zhu C, Wu YF, Zhang L, Tang JB

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Abstract BACKGROUND AIMS: Sustained gene delivery could be particularly necessary in modulating gene expression in healing intrasynovial tendons and may be a promising approach for preventing adhesions. METHODS: Polylactic-co-glycolic acid nanospheres were prepared and were then incorporated with plasmids expressing enhanced green fluorescence protein and miRNA for inhibiting the transforming growth factor-β1 gene expression. RESULTS: We demonstrated that cultured tenocytes could be effectively transfected by means of nanosphere/plasmid. The expression of transforming growth factor-β1 is significantly downregulated in healing chicken flexor tendon treated with nanosphere/plasmid. Histology analysis did not demonstrate any significant inflammation or necrosis in tendons injected with nanosphere/plasmid after surgery. CONCLUSIONS: Nanosphere/plasmid may be a promising non-viral vector for gene therapy of the injured flexor tendon. Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved. This article was published in Cytotherapy and referenced in Journal of Stem Cell Research & Therapy

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