alexa Effective treatment of human breast tumor in a mouse xenograft model with herpes simplex virus type 1 specifying the NV1020 genomic deletion and the gBsyn3 syncytial mutation enabling high viral replication and spread in breast cancer cells.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Genetic Syndromes & Gene Therapy

Author(s): Israyelyan AH, Melancon JM, Lomax LG, Sehgal I, Leuschner C,

Abstract Share this page

Abstract A new oncolytic and fusogenic herpes simplex virus type 1 (HSV-1) was constructed on the basis of the wildtype HSV-1(F) strain. To provide for safety and tumor selectivity, the virus carried a large deletion including one of the two alpha4, gamma(1)34.5, alpha0 genes and the latency-associated transcript region. The gamma(1)34.5 gene, a major neurovirulence factor, was replaced by a gene cassette constitutively expressing the red fluorescent protein gene. Homologous recombination was used to transfer the fusogenic gBsyn3 mutation to the viral genome to produce the OncSyn virus. OncSyn causes extensive virus-induced cell fusion (syncytia) and replicates to higher titers than the parental Onc and HSV-1(F) strains in breast cancer cells. Biochemical analysis revealed that the OncSyn virus retains a stable genome and expresses all major viral glycoproteins. A xenograft mouse model system using MDA-MB-435S-luc (MM4L) human breast cancer cells constitutively expressing the luciferase gene implanted within the interscapular region of animals was used to test the ability of the virus to inactivate breast tumor cells in vivo. Seventy-two mice bearing MM4L breast cancer xenografts were randomly divided into three groups and given two rounds of three consecutive intratumoral injections of OncSyn, inactivated OncSyn, or phosphate-buffered saline 3 days apart. A single round of virus injections resulted in a drastic reduction of tumor sizes (p
  • DOI: 10.1089/hum.2006.145
  • This article was published in Hum Gene Ther and referenced in Journal of Genetic Syndromes & Gene Therapy

    Relevant Expert PPTs

    Relevant Speaker PPTs

    Recommended Conferences

    • 10th International Conference on Genomics and Molecular Biology
      May 21-23, 2018 Barcelona, Spain
    • 5th International Conference on Human Genetics and Genetic Disorders September 21-22,2018 Philadelphia, USA Theme: Sharing Discoveries of the Future Human Genome
      September 21-22,2018 Philadelphia, USA
    • 3rd World Congress on Human Genetics & Genetic Disorders
      October 20-21, 2017 Toronto, Canada

    Relevant Topics

    Peer Reviewed Journals
    Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
    International Conferences 2017-18
    Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

    Contact Us

    Agri & Aquaculture Journals

    Dr. Krish

    1-702-714-7001Extn: 9040

    Biochemistry Journals

    Datta A

    1-702-714-7001Extn: 9037

    Business & Management Journals


    1-702-714-7001Extn: 9042

    Chemistry Journals

    Gabriel Shaw

    1-702-714-7001Extn: 9040

    Clinical Journals

    Datta A

    1-702-714-7001Extn: 9037

    Engineering Journals

    James Franklin

    1-702-714-7001Extn: 9042

    Food & Nutrition Journals

    Katie Wilson

    1-702-714-7001Extn: 9042

    General Science

    Andrea Jason

    1-702-714-7001Extn: 9043

    Genetics & Molecular Biology Journals

    Anna Melissa

    1-702-714-7001Extn: 9006

    Immunology & Microbiology Journals

    David Gorantl

    1-702-714-7001Extn: 9014

    Materials Science Journals

    Rachle Green

    1-702-714-7001Extn: 9039

    Nursing & Health Care Journals

    Stephanie Skinner

    1-702-714-7001Extn: 9039

    Medical Journals

    Nimmi Anna

    1-702-714-7001Extn: 9038

    Neuroscience & Psychology Journals

    Nathan T

    1-702-714-7001Extn: 9041

    Pharmaceutical Sciences Journals

    Ann Jose

    1-702-714-7001Extn: 9007

    Social & Political Science Journals

    Steve Harry

    1-702-714-7001Extn: 9042

    © 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version