Author(s): Amaral L, Lorian V
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Abstract Chlorpromazine (CPZ), at a concentration of 60 micrograms/ml of medium completely inhibited the replication of Escherichia coli. At concentrations below this MIC, CPZ caused transient induction of filamentation, such that by the end of 5 h, all of the cells were filaments, and by the end of 24 h, only rod-shaped E. coli were present. The reversion to normal morphology in the presence of CPZ was not due to either the degradation of CPZ or the selection of CPZ-resistant mutants. The electrophoretic pattern of proteins extracted from isolated cell envelopes of CPZ-induced filaments as well as from E. coli that reverted to normal morphology was distinctly different from that of the controls.
This article was published in Antimicrob Agents Chemother
and referenced in Journal of Medical Microbiology & Diagnosis