alexa Effects of docetaxel, doxorubicin and cyclophosphamide on human breast cancer cell line MCF-7.


Medicinal chemistry

Author(s): Trebunova M, Laputkova G, Slaba E, Lacjakova K, Verebova A

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Abstract BACKGROUND: Cell viability assays are important tools in oncological research. The purpose of this study was to evaluate the effect of docetaxel, doxorubicin and cyclophosphamide on the metabolic activity of MCF-7 breast cancer cell line. MATERIALS AND METHODS: Antiproliferative effect of cytostatics on MCF-7 cells was measured using the standard colorimetric test. The MCF-7 cell line was exposed to cytostatics for 24 hours. The metabolic activity was evaluated over the 24 hours. RESULTS: According to the statistical analysis, the change in the growth rate was significant (p<0.05) for the 120 nM docetaxel and above the 200 nM doxorubicin treatment in comparison with sensitive MCF-7 cells. When considering drugs in combination (60 nM docetaxel with 200 nM doxorubicin and 60 nM docetaxel with 500 nM doxorubicin) after the addition of 600 nM cyclophosphamide, we found a statistically significant decrease of metabolic activity of the MCF-7 cell line (p<0.05). CONCLUSION: Cyclophosphamide at 600 nM seems to enhance the influence of docetaxel when combined with doxorubicin.
This article was published in Anticancer Res and referenced in Medicinal chemistry

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