alexa Effects of estrogen and estrogen receptor in normal human melanocytes.
Dermatology

Dermatology

Journal of Pigmentary Disorders

Author(s): Jee SH, Lee SY, Chiu HC, Chang CC, Chen TJ, Jee SH, Lee SY, Chiu HC, Chang CC, Chen TJ

Abstract Share this page

Abstract Normal human melanocytes were cultured selectively with F12 culture medium supplemented with growth hormones, phorbol ester and 1\% of fetal calf serum. The estrogen receptors were analyzed using hydroxylapatite-column assay with tritiated 17-beta-estradiol as the binding ligand. Phenol red- free medium was used when the changes in cell numbers, melanin content and tyrosinase were assessed after incubating with physiological concentration of 17-beta estradiol (10(-12) and 10(-9) M). It was found that the melanocytes contained both cytosol (5.42 +/- 1.11 fmol/mg protein) and nuclear (59.13 +/- 17.12 fmol/mg protein) estrogen receptor. In response to estradiol, the cell number increased but both the melanin content and the tyrosinase activity decreased in a dose related pattern. These data suggested the presence of estrogen receptor with biological function in normal human melanocytes. This article was published in Biochem Biophys Res Commun and referenced in Journal of Pigmentary Disorders

Relevant Expert PPTs

Relevant Speaker PPTs

  • Hana Zelenkova
    Therapy with immune modulators (cyclosporine A) in dermatology (focusing on psoriasis, atopic eczema, allergic vasculitis, and chronic urticaria)
    PPT Version | PDF Version

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords