alexa Effects of estrogen and estrogen receptor in normal human melanocytes.


Journal of Pigmentary Disorders

Author(s): Jee SH, Lee SY, Chiu HC, Chang CC, Chen TJ, Jee SH, Lee SY, Chiu HC, Chang CC, Chen TJ

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Abstract Normal human melanocytes were cultured selectively with F12 culture medium supplemented with growth hormones, phorbol ester and 1\% of fetal calf serum. The estrogen receptors were analyzed using hydroxylapatite-column assay with tritiated 17-beta-estradiol as the binding ligand. Phenol red- free medium was used when the changes in cell numbers, melanin content and tyrosinase were assessed after incubating with physiological concentration of 17-beta estradiol (10(-12) and 10(-9) M). It was found that the melanocytes contained both cytosol (5.42 +/- 1.11 fmol/mg protein) and nuclear (59.13 +/- 17.12 fmol/mg protein) estrogen receptor. In response to estradiol, the cell number increased but both the melanin content and the tyrosinase activity decreased in a dose related pattern. These data suggested the presence of estrogen receptor with biological function in normal human melanocytes. This article was published in Biochem Biophys Res Commun and referenced in Journal of Pigmentary Disorders

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