Author(s): Saim Ozdamar
AbstractPurpose: We targeted to investigate the effects of paclitaxel and cisplatin treatments, both solely and in combination with each other, on ovarian reserves in rats. Materials and methods: Forty female Wistar albino rats were divided into 4 groups as to include 10 rats in each. While 7.5 mg/kg paclitaxel was given to group II, 5mg/kg cisplatin was administered to group III, 7.5 mg/kg paclitaxel and 5 mg/kg cisplatin combination therapy was applied to group IV via intraperitoneal route in a sterile way at single dose. Group I, which was assigned to be the control group, was subjected to intraperitoneal sterile saline in equal volumes. One week after the chemotherapy, ovaries of all the rats were oophorectomized. Hematoxylin-eosin stain was used to determine the follicles. Results: Number of primordial, primary, and secondary follicles were found to show a statistically significant decrease (p<0.001). Most significant drop in number of follicles was determined to be in group 4. Conclusion: Single and combined use of paclitaxel and cisplatin caused a decrease in number of primordial, primary, secondary, and tertiary follicles. Most significant drop regarding the number of follicles, was found to be in the combined chemotherapy group. Therefore, single-agent chemotherapy regimes may be considered as an alternative for patients asking for fertility.