Author(s): Wang ZY, Lin JM, Xu ZF
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Abstract OBJECTIVE: To develop a new technique for efficient and rapid non-test tube cloning of the medicinal and energy- producing plant Jatropha curcas. METHODS: Using the mini-stem fragment (2-3 cm) of Jatropha curcas with merely one axillary bud as the explant, the effect of an auxin IBA concentration on the plantlet regeneration was studied. RESULTS AND CONCLUSION: When treated with 1 mg/LIBA for 1h, the explants showed the most rapid propagation. The mini-stem fragments high root regeneration ratio (96.7\%), short root regeneration period (18.2-/+2.0 d), large number of new roots per explant (6.3-/+1.8), and long total root length (6.8-/+3.5 cm), demonstrating that this technique can be a simple and efficient method for rapid non-test tube cloning of Jatropha curcas of potential industrial value.
This article was published in Nan Fang Yi Ke Da Xue Xue Bao
and referenced in Journal of Bioremediation & Biodegradation