alexa Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Genetic Syndromes & Gene Therapy

Author(s): Behr JP, Demeneix B, Loeffler JP, PerezMutul J

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Abstract A general and efficient transfection procedure, based on compacted lipopolyamine-coated plasmids, has been developed. The active species is obtained by simple addition of excess synthetic lipospermine solution to the DNA and binds within minutes to the cell membrane. This technique has been developed on endocrine cells of the intermediate lobe of the pituitary as a general tool for physiological work on primary cells; it is not toxic and does not interfere with physiological regulations in melanotrope cells. A variety of eukaryotic cell cultures also have been transfected with success for transient and stable expression.
This article was published in Proc Natl Acad Sci U S A and referenced in Journal of Genetic Syndromes & Gene Therapy

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