Author(s): Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM, Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM
Abstract Share this page
Abstract Electrospray ionization has recently emerged as a powerful technique for producing intact ions in vacuo from large and complex species in solution. To an extent greater than has previously been possible with the more familiar "soft" ionization methods, this technique makes the power and elegance of mass spectrometric analysis applicable to the large and fragile polar molecules that play such vital roles in biological systems. The distinguishing features of electrospray spectra for large molecules are coherent sequences of peaks whose component ions are multiply charged, the ions of each peak differing by one charge from those of adjacent neighbors in the sequence. Spectra have been obtained for biopolymers including oligonucleotides and proteins, the latter having molecular weights up to 130,000, with as yet no evidence of an upper limit.
This article was published in Science
and referenced in Advanced Techniques in Biology & Medicine