alexa Elevated Expression of indoleamine 2,3-dioxygenase (IDO) and accumulation of kynurenic acid in the pathogenesis of STZ-induced diabetic cataract in Wistar rats.
Diabetes & Endocrinology

Diabetes & Endocrinology

Journal of Diabetic Complications & Medicine

Author(s): Kanth VR, Kanth VR, Lavanya K, Srinivas J, Raju TN

Abstract Share this page

Abstract PURPOSE: Glycated lens proteins are capable of producing reactive oxygen species (ROS), which, in turn, can oxidize tryptophan (Trp) into kynurenines. Indoleamine 2,3-dioxygenase (IDO), which is expressed in many tissues and which is inducible by interferon-gamma (IFN-gamma), is able to oxidize Trp into kynurenines. These kynurenines can modify lens proteins and, in fact, kynurenine adducts are markedly increased in lenses with age-related nuclear cataract. Therefore, it has been suggested that lenticular IDO is involved in diabetic cataractogenesis. The aim of the present study was to examine the possible role(s) of IDO in streptozotocin (STZ)-induced diabetic cataract in rats. METHODS: Diabetic cataract was induced in male Wistar-NIN rats by IP injection of STZ (34 mg/kg body wt). Slit lamp biomicroscopy was used to monitor progression of the resulting hyperglycemia-induced cataract. Treated and control rats were sacrificed at 30 and 60 days, at which times changes in lenticular levels of IDO activity, IDO mRNA, IFN-gamma mRNA (IDO inducer), Trp, kynurenic acid (KYNA), oxidative stress markers (malondialdehyde and carbonyls), antioxidant (reduced glutathione), antioxidant enzymes (glutathione peroxidase and superoxide dismutase), and polyol enzymes (aldose reductase and sorbitol dehydrogenase) were determined and compared. RESULTS: Cataract was observed to begin at 30 days after STZ treatment, and mature cataract was observed 60 days after STZ treatment. Lenticular levels of IDO activity, IDO mRNA, IFN-gamma mRNA, Trp, and KYNA increased significantly at 30 days and remained elevated through 60 days. Significant increases were also observed in levels of oxidative stress markers, antioxidant enzymes, and polyol enzymes at 30 and 60 days after STZ treatment. However, the level of reduced GSH decreased by approximately 50\% at both points of determination. CONCLUSIONS: Production of IDO was induced in STZ-induced diabetic cataractous lenses, possibly by locally produced IFN- gamma. IDO-mediated oxidation of Trp may partly explain the increase in lens KYNA and may thus be implicated in cataractogenesis in concert with the non-enzymic oxidation of Trp by glycated lens proteins. This article was published in Curr Eye Res and referenced in Journal of Diabetic Complications & Medicine

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

OMICS International Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version