Author(s): Bartel P, Chien CT, Sternglanz R, Fields S
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Abstract The two-hybrid system is a genetic method to identify proteins that interact with a specific target protein, which is expressed in yeast as a hybrid with a DNA-binding domain. Use of this method entails transforming yeast, both with this DNA-binding domain hybrid and with a library of activation domain hybrids, followed by screening for transcriptional activation of a reporter gene. In addition to proteins that interact with the target protein, a number of false positives are identified. We provide possible explanations for these false positives along with rapid assays to eliminate them.
This article was published in Biotechniques
and referenced in Journal of Biodiversity, Bioprospecting and Development