Author(s): Dugo P, Skerikov V, Kumm T, Trozzi A, Jandera P,
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Abstract A novel approach for carotenoid analysis has been developed. Orange essential oil and juice carotenoids were separated by means of comprehensive dual-gradient elution HPLC, using normal phase with a microbore silica column in the first dimension (first D), reversed phase with a monolithic C18 column in the second dimension (second D), and a 10-port switching valve as an interface. An on-line photodiode array detector was used in order to obtain absorption spectra. Peak identification was obtained by combining retention data with the UV-visible spectra.
This article was published in Anal Chem
and referenced in Journal of Chromatography & Separation Techniques