alexa Endogenous electrical currents and the resultant voltage gradients in the chick embryo.
General Science

General Science

Biological Systems: Open Access

Author(s): Hotary KB, Robinson KR, Hotary KB, Robinson KR

Abstract Share this page

Abstract We have studied some of the electrophysiological properties of 2 1/2- to 4-day-old (stage 14-22) chick embryos. Using a recently developed two-dimensional vibrating probe, large currents were found to exit the posterior intestinal portal (p.i.p.) during the period of tail gut reduction. During this period, epithelial cells lining cloacal regions of the hindgut are dying, thus creating a low-resistance pathway for current flow out of the embryo. Currents entered the intact epithelium over other regions of the embryo. The outward currents at the p.i.p. were first detected at stage 15 and reached their average maximum current density of 112 +/- 10 microA/cm2 at stage 17. After stage 17, the magnitude of the currents decreased, dropping to 16 +/- 0.3 microA/cm2 by stage 22. The currents were reversibly reduced by about 50\% when Na+ was replaced by choline in the bathing solution. The magnitude of the currents leaving the p.i.p. suggested the existence of a measurable intraembryonic voltage gradient. The transepithelial potential (TEP) of stage 14-21 embryos was measured lateral to the neural tube through the dorsal ectoderm. For all stages, the combined average TEP was 16 +/- 0.5 mV. Differences in the TEP between various regions of the embryo were used to calculate an intraembryonic voltage gradient. At stage 14, before outward current was found at the p.i.p., no significant intraembryonic voltage gradient was detected. At stage 17, when the outward current at the p.i.p. was maximum, a voltage gradient of 21 +/- 5 mV/mm (mean +/- SEM; N = 6) was measured in the caudal end of the embryo. This gradient in some cases was as steep as 33 mV/mm. This is well above the minimum level needed to affect the direction of embryonic cell migration in vitro. We hypothesize that this endogenous electrical field acts as a directional cue for neural crest cell movements in the developing chick embryo.
This article was published in Dev Biol and referenced in Biological Systems: Open Access

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

OMICS International Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version