Author(s): Chen CY, Chen YC, Huang HC, Huang CC, Lee WL, , Chen CY, Chen YC, Huang HC, Huang CC, Lee WL, , Chen CY, Chen YC, Huang HC, Huang CC, Lee WL,
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Abstract Microalgae have emerged as promising resources for highly unsaturated fatty acids. In this study, an indigenous microalga identified as Nannochloropsis oceanica CY2 was grown photoautotrophically to produce eicosapentaenoic acid (EPA; 20:5, n-3). Specific engineering strategies were employed to stimulate EPA accumulation in the microalgal cells. The results show that BG-11 was the most effective medium to grow N. oceanica CY2, giving an EPA content and biomass concentration of 2.38\% (per dry cell weight) and 1.53 g/l. The EPA content nearly doubled when using the optimal nitrogen source (NaNO3) at a concentration of 1.50 g/l. The illumination system also markedly affected the EPA content for the photoautotrophic microalga. When the microalgal culture was illuminated with a red LED, an impressively high EPA content of 5.5\% was obtained. Finally, using semi-batch cultures operations with LED-blue illumination, the EPA content of N. oceanica CY2 was stably maintained at 5.0\%. Copyright © 2013 Elsevier Ltd. All rights reserved.
This article was published in Bioresour Technol
and referenced in Current Synthetic and Systems Biology