alexa Enzymatic iron oxidation by Leptothrix discophora: identification of an iron-oxidizing protein.


Journal of Microbial & Biochemical Technology

Author(s): Corstjens PL, de Vrind JP, Westbroek P, de Vrindde Jong EW, Corstjens PL, de Vrind JP, Westbroek P, de Vrindde Jong EW

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Abstract An iron-oxidizing factor was identified in the spent culture medium of the iron- and manganese-oxidizing bacterial strain Leptothrix discophora SS-1. It appeared to be a protein, with an apparent molecular weight of approximately 150,000. Its activity could be demonstrated after fractionation of the spent medium by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A spontaneous mutant of L. discophora SS-1 was isolated which excreted neither manganese- nor iron-oxidizing activity, whereas excretion of other proteins seemed to be unaffected. Although the excretion of both metal-oxidizing factors was probably linked, the difference in other properties suggests that manganese and iron oxidation represent two different pathways. With a dot-blot assay, it was established that different bacterial species have different metal-oxidizing capacities. Whereas L. discophora oxidized both iron and manganese, Sphaerotilus natans oxidized only iron and two Pseudomonas spp. oxidized only manganese.
This article was published in Appl Environ Microbiol and referenced in Journal of Microbial & Biochemical Technology

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