alexa Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.
Nursing

Nursing

Journal of Nursing & Care

Author(s): Hamady M, Walker JJ, Harris JK, Gold NJ, Knight R

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Abstract We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92\% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
This article was published in Nat Methods and referenced in Journal of Nursing & Care

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